A novel method for "Wet" SEM.

نویسندگان

  • Iris Barshack
  • Juri Kopolovic
  • Yehuda Chowers
  • Opher Gileadi
  • Anya Vainshtein
  • Ory Zik
  • Vered Behar
چکیده

Progress in the processing of wet tissues, without the need of fixation and complex preparation procedures, may facilitate the microscopic examination of tissues and cells. Microscopic examination of tissues is a central tool in clinical diagnosis as well as in diverse areas of research. The authors present the application of Wet SEM, a technology for imaging fully hydrated samples at atmospheric pressure in a scanning electron microscope (SEM). The technique is based on 2 principles. First, samples are imaged in sealed specimen capsules and are separated from the evacuated interior of the electron microscope by a thin, electron-transparent partition membrane that is strong enough to sustain a 1-atm pressure difference. Second, imaging is done in a SEM, based on detection of backscattered electrons, which penetrate a few microns into the specimen and thus give information on the cellular level.

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عنوان ژورنال:
  • Ultrastructural pathology

دوره 28 1  شماره 

صفحات  -

تاریخ انتشار 2004